Lab test

Cortisol AM & PM Test - Cortisol Levels

This blood test measures the hormone cortisol in the morning and again in the afternoon to show how its level changes across the day.

Typical price

From $129

Results

1-3 business days

Preparation

See preparation notes below

Sample

Blood draw or sample collection at a local center

This blood test measures the hormone cortisol in the morning and again in the afternoon to show how its level changes across the day. Cortisol is produced by the adrenal glands and is central to the body's stress response, blood sugar regulation, blood pressure, and immune activity. Because cortisol normally peaks in the early morning and declines later in the day, collecting two samples helps reveal the daily rhythm rather than a single snapshot. Practitioners order it to investigate persistent fatigue, unexplained weight change, mood or sleep concerns, and to help evaluate suspected adrenal underactivity or overactivity. Results are interpreted alongside symptoms and the timing of each collection.

• Cortisol (morning) Cortisol level measured in the morning, when it is typically at its highest.
• Cortisol (afternoon) Cortisol level measured later in the day, when it is typically lower.

• Adults with ongoing fatigue, low energy, or difficulty waking
• People being evaluated for possible adrenal gland concerns
• Individuals with unexplained weight changes, blood pressure changes, or mood and sleep disturbance
• Those being monitored after earlier abnormal adrenal or pituitary findings

• Two blood draws are needed, one in the morning and one in the afternoon
• Morning samples are usually collected shortly after waking, as directed by the laboratory
• Tell the technician about medications that affect hormones, including steroid medications
• Follow any timing or fasting instructions provided with your order

Results show cortisol at two times of day and are assessed against reference ranges for each collection time. A morning value that is unusually low may point toward reduced adrenal output, while a persistently elevated level may suggest increased adrenal activity or stress-related causes. The relationship between the two samples matters, since a loss of the normal daily decline can be meaningful. Because many factors influence cortisol, results should be interpreted by a healthcare provider alongside symptoms and other testing.

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