Lab test

Nicotine Urine Test - Nicotine Testing

This urine test measures nicotine and its metabolites, including cotinine, to assess recent exposure to tobacco or other nicotine products.

Typical price

From $159

Results

1-3 business days

Preparation

See preparation notes below

Sample

Blood draw or sample collection at a local center

This urine test measures nicotine and its metabolites, including cotinine, to assess recent exposure to tobacco or other nicotine products. It is commonly used to monitor the success of smoking cessation programs, check for nicotine use in clinical or wellness settings, and detect passive exposure. Because nicotine is broken down quickly while cotinine lingers longer, the combination gives a practical picture of recent use. The test does not measure the health effects of tobacco; it shows whether nicotine has been used.

• Nicotine The parent compound in tobacco and nicotine products, detectable briefly after use.
• Cotinine The main nicotine metabolite and the primary marker of recent nicotine exposure in urine.

• People enrolled in a smoking cessation program
• Individuals whose provider is monitoring nicotine abstinence
• Patients being assessed for nicotine exposure
• People concerned about secondhand smoke exposure

• No special preparation is usually needed
• Avoid nicotine products before collection if abstinence is being checked
• Disclose nicotine replacement products and medications
• Follow any specific instructions from your program

Detectable nicotine or cotinine indicates recent nicotine exposure from smoking, vaping, smokeless tobacco, nicotine replacement, or secondhand smoke. Lower levels may reflect lighter or passive exposure. Because detection times vary with use and individual metabolism, results are interpreted in context. Discuss your urine nicotine result and what it means for your health goals with a healthcare provider.

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